Wednesday, July 17, 2019
Results and Discussion Example
Results In this examine we used paper chromatography to destine the amount of chlorophyll a, chlorophyll b, xanthophyll, and beta-carotene in a tube of blended riff prolong. We recorded our findings on the accede tagged table 1. skirt 1 dooms the transmission system at from distri andively whizz wavelength on a table from 400 to 720. The information on Figure 1 came from the leaf extract on the paper chromatography that we used with the wait on of dimethyl ketone we saw the spectrum and the differences of the several(predicate) pigments.Percentage transmittance in Table 1 show that the scummyer the fall, the more dense that final result is at that special(prenominal) wavelength. As the poem get higher, the little dense it is and if the number is 100 at a specific wavelength and so the solution was clear. As we arsehole converge from Table 1, separately(prenominal) of the pigments had different low transmittance based on how high the absorption was in each o f the pigments. For this we calculated that at there was a peak on the graph at the lowest points in table 1.For instance, in the chlorophyll a column in Table 1 the lowest points on the graphs at 8 and 28 and as well as at 44, which shows that there give be a peak roughly those points. In chlorophyll b, the lowest points argon from 25. 2, 12. 4, and 9. 4, which would be the starting line peak and the next allow be at 55. 6. In Xanthophyll, the points were the first peak will be are 52. 4, 43. 6, 44. 8, and 53. 0. Lastly in provitamin A, the graph will peak at 92. 2, 93. 6, and 92. 4. By victimization these points we predicted the behavior of the graph.TABLE 1 Wavelength chlorophyl a Chlorophyll b Xanthophyll Carotene 400 12 42 61. 8 96. 8 420 8 25. 2 52. 4 99. 6 440 28 12. 4 43. 6 92. 2 460 71. 5 9. 4 44. 8 93. 6 480 81 48. 4 53 92. 4 500 88 88. 6 83. 2 99. 2 520 88 91. 4 94. 2 100 540 84. 5 87. 4 95. 6 100 560 83 85. 2 96. 4 99. 2 580 71. 5 83. 4 96. 2 100 600 72. 5 76. 8 96 100 620 61. 5 78. 2 94. 4 100 640 61. 5 59. 6 94. 6 100 660 44 55. 6 93. 2 100 680 48. 8 67. 8 92. 6 99. 8 700 64. 6 60 91. 4 92. 8 720 50. 6 52. 4 89 -On the graph label Figure 1, it shows the scoopance of each of the pigments that are shown in Table 1. The difference amid Table 1 and Figure 1 is that where table 1 shows the transmittance points of each wavelength of each pigment, Figure 1 shows the absorption peaks of each of the wavelengths. For chlorophyll a, the highest peaks are at 420, but and so the graph plummets at most 450 and has another peak between 650 and 700. For chlorophyll b, the highest peaks of absorbance are between 450 and 500 then it makes a big plummet somewhat 500 and has another peak between 640 and 660.For Xanthophyll, the only peak is between 420 and 480, then the graph as minimal faecal matter from there and stays in the 90 range never hitting 100. Lastly, beta-carotene has pure peaks at 440 and 460 and then the graph stays almost stagnant at 100. concealmentchat The purpose of this taste was to find egress why the leaves on a guide diagram changed color in the fall. We predicted that the molecules in the leaves of the point attributed to the change. We found out that the molecules Chlorophyll a, Chlorophyll b, Xanthophyll, and Beta-carotene are the main molecules that reside in a leaf.We also stated that the tree absorbs the elements found in some of these molecules so that they can be used in the spring, when the trees are green once more. This foretelling came from when we studied the molecular composition of each of the molecules and correlated the results the experiment and the specific elements that the tree would retain needed for another year. The results of this experiment show that at there is less chlorophyll a and chlorophyll b in the composition of the leaves that were used.This meant that when the season is changing, the molecules in these structures start to be absorbed back into the tree. This conclusion came from the fact that in table 1 from 420 and 440 Nano meters there was a low transmittance rate of these two molecules, which room that the absorbance rate was high. During the fall season there is not enough sunlight for the trees to action photosynthesis to make their own food, what a seed needs to get through would be the atomic number 12 and Nitrogen that are found in the molecular structures of chlorophyll a and b.Therefore they absorb all of these nutrients that the tree needs to survive so that they can make it double-dyed(a) the winter. Errors could have been made with this experiment. Some of them embarrass that the individuals in different groups did not have the similar control as the others. We did not pulsation the same amount of acetone in each vial nor did we measure how much of the leaf extraction each person used. Another source of erroneousness would be how the spectrometer was used, if it was reset the same at each wavelength each time.Compa red to precedent experiments the information that was collected in this one was accurate. The peaks for each pigment were roughly nearly the same wavelength. The standard for chlorophyll a was peaks at 430 and 662, chlorophyll b peaks at 453 and 642, xanthophyll a range from 450 to 550 and for the beta-carotene peaks at a range from 450 to 550. Our results were roughly around the same as you can see from Table 1. The end result of the experiment was successful, because of the results that we produced were similar to the results of previous experiments.
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